Laboratory Techniques in Thrombosis — a Manual

✍ Scribed by J. Gram, J. Jespersen (auth.), J. Jespersen, R. M. Bertina, F. Haverkate (eds.)

• Publisher: Springer Netherlands
• Year: 1999
• Tongue: English
• Leaves: 307
• Edition: 2
• Category: Library

✦ Synopsis

The first edition of this manual appeared in 1992 and was entitled ECAT Assay Procedures. It was the result of a unique cooperation between experts brought together by the European Concerted Action on Thrombosis and Disabilities (ECAT). The Concerted Action was at that time under the auspices of the Commission of the European Union. The second edition, like the first edition, deals with diagnostic tests within the field of thrombosis. However, the second edition has a broader scope because it is no longer limited by the frontiers of ECAT. Experts allover the world, in and outside ECAT, have contributed to this edition. The editors are very grateful for their contributions. The need for a new edition is obvious. Since 1992 new assays have been introduced for research, diagnosis, and therapy of thrombosis; for other assays improvements have been suggested, while a few others became redundant. The editors waived the radioimmunoassays of ~-thrombog1obulin and platelet factor 4 due to the fact that the kits required for these assays are rarely, or no longer, available. Also the PAI-1 activity assay was waived as it is liable to many inconsistencies and to large variations. A list of names and addresses of manufacturers marketing the kits and reagents has been compiled, together with a list of the recommended nomenclature of quantities in thrombosis and haemostasis, in order to facilitate the use of the updated version. These lists have been carefully compiled by Johannes J. Sidelmann, PhD, Department of Clinical Biochemistry in Esbjerg, Denmark.

✦ Table of Contents

Front Matter....Pages i-xviii
Introduction to laboratory assays in haemostasis and thrombosis....Pages 1-7
Good medical laboratory services — guidelines....Pages 9-19
Blood collection and sample preparation: pre-analytical variation....Pages 21-28
Quality assessment of haemostatic assays and external quality assessment schemes....Pages 29-36
Activated partial thromboplastin time (APTT)....Pages 37-44
Prothrombin time (PT)....Pages 45-61
Endogenous thrombin potential....Pages 63-78
Fibrinogen....Pages 79-88
Activated factor VII....Pages 89-97
Factor VII activity and antigen....Pages 99-106
Factor VIII clotting activity....Pages 107-113
Von Willebrand factor....Pages 115-119
Antithrombin activity and antigen....Pages 121-128
Protein C activity and antigen....Pages 129-139
Protein S antigen....Pages 141-151
Protein S activity....Pages 153-162
Activated protein C (APC) resistance....Pages 163-169
Tissue factor pathway inhibitor (TFPI)....Pages 171-181
Lupus anticoagulant....Pages 183-187
Heparin cofactor II....Pages 189-198
Fibrinopeptide A (FPA)....Pages 199-207
Thrombin-antithrombin (TAT) complexes....Pages 209-216
Prothrombin fragment F1+2....Pages 217-222
Tissue-type plasminogen activator (t-PA) activity....Pages 223-230
Tissue-type plasminogen activator antigen (t-PA Ag)....Pages 231-237
Plasminogen activator inhibitor-1 (PAI-1) antigen....Pages 239-245
Plasminogen activity....Pages 247-255
Plasmin inhibitor activity (previously α 2 -antiplasmin)....Pages 257-264
Plasmin-α 2 -antiplasmin complexes (plasmin—plasmin inhibitor complexes)....Pages 265-274
Soluble fibrin and degradation products of fibrinogen (FgDP), fibrin (FbDP; D-dimer) and total of FgDP and FbDP (TDP)....Pages 275-284
Venous occlusion test in fibrinolysis assays....Pages 285-292
Back Matter....Pages 293-308

✦ Subjects

Hematology; Laboratory Medicine; Medical Biochemistry