Laboratory Techniques in Membrane Biophysics: An Introductory Course

✍ Scribed by W. McD. Armstrong, K. Baumann, P. C. Caldwell, T. W. Clarkson, J. Dudel, B. Frankenhaeuser, E. Frömter, G. Gardos, B. Z. Gilzburg, J. F. Hoffman, A. Kepes, R. D. Keynes, A. Kleinzeller, P. G. Kostyuk, A. Kotyk, A. A. Lev, B. Lindemann, P. Mueller, H. Muth, W. Nonner, E. Oberhausen, H. Passow, A. Rothstein, D. O. Rudin, R. Stämpfli, T. Teorell, K. J. Ullrich, N. A. Walker, W. Wilbrandt (auth.), H. Passow, R. Stämpfli (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1969
• Tongue: English
• Leaves: 206
• Edition: 1
• Category: Library

✦ Synopsis

The present manual contains a collection of laboratory instructions used during an international training course on membrane biophysics which was held at Homburg in the fall of 1966. The selection of the topics dealt with in the various chapters depended on the scientific interest of the available teachers and on the availability of the necessary equipment in our laboratories. Thus, the material included in this volume does not add up to a systematic course in membrane biophysics. Instead it represents a more fortuitous collection of laboratory problems. In addition, some authors place more emphasis on teaching the more technical aspects of a method whereas others are primarily concerned with the demonstra­ tion of a significant biological phenomenon. Nevertheless, in spite of such differences of emphasis and a somewhat haphazard choice of a few methods and phenomena among many others of similar importance, it was felt that the publication of the material is desirable. Since no other laboratory manual exists so far, the present laboratory problems which were tested in actual practice may serve as a useful basis for the shaping of further training courses or for laboratory courses for graduate students in biophysics and physiology. Our thanks are due to the authors and the publisher who were patient and kind enough to cooperate with the editors during the long period between the end of the course and the appearance of the book.

✦ Table of Contents

Front Matter....Pages I-VIII
Measurement of Hydraulic Conductivity and Reflexion Coefficients of a Plant Cell Membrane by means of Transcellular Osmosis....Pages 1-8
Flux Measurements in Erythrocytes....Pages 9-20
Ion Permeability of Erythrocyte Ghosts....Pages 21-27
Countertransport in Human Red Blood Cells....Pages 28-33
Kinetics of Cation Transport in Yeast....Pages 34-48
Bacterial Permeases....Pages 49-62
The Exchange of 22 Na between Frog Sartorius Muscle and the Bathing Medium....Pages 63-68
Determination of Intracellular Ionic Concentrations and Activities....Pages 69-84
Experiments on Na Transport of Frog Skin Epithelium....Pages 85-105
Micropuncture and Microanalysis in Kidney Physiology....Pages 106-129
Oscillatory Phenomena in a Porous, Fixed Charge Membrane....Pages 130-140
Bimolecular Lipid Membranes: Techniques of Formation, Study of Electrical Properties, and Induction of Ionic Gating Phenomena....Pages 141-156
Dissection of Single Nerve Fibres and Measurement of Membrane Potential Changes of Ranvier Nodes by means of the Double Air Gap Method....Pages 157-166
Ionic Currents in the Myelinated Nerve Fibre....Pages 167-170
A New Voltage Clamp Method....Pages 171-175
Electrical Activity of Synapses. The Crayfish Neuromuscular Junction....Pages 176-180
Back Matter....Pages 181-201

✦ Subjects

Biochemistry, general;Human Physiology;Biophysics and Biological Physics