Laboratory experiments on the regulation of pyruvate kinase isoenzymes
β Scribed by E Hannappel; W Fischer; K Brand
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 178 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0307-4412
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The biosynthesis of the enzyme pyruvate kinase (E.C. 2.7.1.40) of Alcaligenes eutrophus (Hydrogenomonas eutropha) H 16 was influenced by the carbon and energy source. After growth on gluconate the specific enzyme activity was high while acetate grown cells exhibited lower activities (340 and 55 mumo
Escherichia coli strains devoid of one or both of the two pyruvate kinase isoenzymes (PKA and PKF), were grown on minimal media in batch fermentations. The strain lacking both PKs showed a 28% decrease on its specific growth rate when compared to the wild type. However, protein and CO 2 yields did n