๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Laboratory automation of high-pressure liquid chromatography

โœ Scribed by William F. Beyer; David D. Gleason


Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
413 KB
Volume
64
Category
Article
ISSN
0022-3549

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A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated fr