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Labelling of the catalytic site of lysozyme

โœ Scribed by Gian-Luigi Rossi; Eggehard Holler; Suriender Kumar; John A. Rupley; George P. Hess

Book ID
118851523
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
511 KB
Volume
37
Category
Article
ISSN
0006-291X

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Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme
โœ Anton V. Filikov; John R. Jones; Nikolai F. Myasoedov; E. Sally Ward ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 438 KB ๐Ÿ‘ 1 views

## Abstract Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemica