Labelling of human serum albumin with 99mTc using Sn(II) citrate

✍ Scribed by M. T. El-Kolaly; A. S. El-Wetery

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: French
• Weight: 489 KB
• Volume: 28
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580280310

✦ Synopsis

Abstract

Labelling of human serum albumin (HSA) with 99m Tc, at neutral pH, using Sn(II) citrate as reducing agent is described. At pH 7.4, more than 98% binding efficiency for 99m Tc‐HSA is achieved. ^99m^Tc‐Sn‐citrate with high protein binding capacity is first formed as an intermediate complex and in the presence of albumin, a more stable ^99m^Tc‐HSA complex is formed. ^99m^Tc‐HSA complex is formed by ligand exchange and 30 min reaction time is sufficient for the complex formation of ^99m^Tc‐HSA. Biodistribution in normal mice at 30 min obtained for ^99m^Tc‐HSA prepared by Sn(II) citrate showed 30.3% injected dose in blood compared to 32.9–34.5% for ^99m^Tc‐HSA prepared by Sn(II)Cl~2~. The presence of citrate stabilizes Sn(II) ions towards oxidation and hydrolysis. The addition of ascorbic acid did not increase the percent yield of ^99m^Tc‐HSA and has adverse effect on its biological distribution.