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Labeling of human C-peptide by conjugation with N-succinimidyl-4-[18F]fluorobenzoate

✍ Scribed by Anna Fredriksson; Peter Johnström; Sharon Stone-Elander; Per Jonasson; Per-Åke Nygren; Karin Ekberg; Bo-Lennart Johansson; John Wahren

Publisher
John Wiley and Sons
Year
2001
Tongue
French
Weight
167 KB
Volume
44
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

We have labeled proinsulin connecting peptide (C‐peptide) with fluorine‐18 (t~½~=109.7 min) in order to perform in vivo biodistribution and pharmacokinetic studies with position emission tomography (PET). This study reports the optimization of the conjugation labeling in the N‐terminal with N‐succinimidyl‐4‐[^18^F]fluorobenzoate ([^18^F]SFB). In preparative runs N‐4‐[^18^F]fluorobenzoyl‐C‐peptide ([^18^F]FB‐C‐peptide) was produced in 8–12% decay‐corrected yields, counted from resolubilized [^18^F]F^−^, in less than 5 h. The specific radioactivity of [^18^F]FB‐C‐peptide, determined using ELISA for one of the preparations, was around 70 GBq/μmol at end of synthesis. Copyright © 2001 John Wiley & Sons, Ltd.

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