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Label-Free Microscale Thermophoresis Discriminates Sites and Affinity of Protein–Ligand Binding

✍ Scribed by Susanne A. I. Seidel; Dr. Christoph J. Wienken; Sandra Geissler; Dr. Moran Jerabek-Willemsen; Dr. Stefan Duhr; Alwin Reiter; Prof. Dirk Trauner; Prof. Dieter Braun; Dr. Philipp Baaske


Book ID
115550004
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
901 KB
Volume
51
Category
Article
ISSN
0044-8249

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Binding affinity and binding-pocket polarity is determined for intracellular fatty acid-binding protein (FABP) from aerobic muscle of Chaenocephalus aceratus, the Antarctic icefish, and from rat heart. FABPs bind fatty acids via weak-bond forces (both ionic and hydrophobic), and these bond forces ar