Label-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy
✍ Scribed by Samir Awasthi; Dennis L. Matthews; Ronald A. Li; Nipavan Chiamvimonvat; Deborah K. Lieu; James W. Chan
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 755 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1864-063X
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✦ Synopsis
Abstract
Pluripotent stem cell‐derived cardiomyocytes (PSC‐CMs) are a potentially unlimited source of cardiomyocytes (CMs) for cardiac transplantation therapies. The establishment of pure PSC‐CM populations is important for this application, but is hampered by a lack of CM‐specific surface markers suitable for their identification and sorting. Contemporary purification techniques are either non‐specific or require genetic modification. We report a second harmonic generation (SHG) signal detectable in PSC‐CMs that is attributable to sarcomeric myosin, dependent on PSC‐CM maturity, and retained while PSC‐CMs are in suspension. Our study demonstrates the feasibility of developing a SHG‐activated flow cytometer for the non‐invasive purification of PSC‐CMs. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)