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L-α-Amino-β-thio-ϵ-caprolactam, a new sulfur-containing Substrate for α-amino-ϵ-caprolactam racemase

✍ Scribed by Syed Ashrafuddin Ahmed; Nobuyoshi Esaki; Hidehiko Tanaka; Kenji Soda


Book ID
115916075
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
360 KB
Volume
174
Category
Article
ISSN
0014-5793

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Amino acid amide racemizing activity was discovered in ␣-amino--caprolactam (ACL) racemase (EC 5. 1. 1. 15) from Achromobacter obae. The nucleotide sequence of 1305 bp ACL racemase gene was designed for expression in Escherichia coli and synthesized in a reaction with 46 oligonucleotides by assembly