Knockouts of Se-glutathione peroxidase-1 and Cu,Zn superoxide dismutase exert different impacts on femoral mechanical performance of growing mice
✍ Scribed by Xiaodan Wang; Elizabeth A. Gillen; Marjolein C. H. van der Meulen; Xin Gen Lei
- Book ID
- 102515058
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 197 KB
- Volume
- 52
- Category
- Article
- ISSN
- 1613-4125
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✦ Synopsis
Abstract
The objective of this study was to determine the impact of knockout of Cu,Zn‐superoxide dismutase (SOD1) and Se‐glutathione peroxidase‐1 (GPX1) on murine bone biomechanical properties. Femora samples were collected from wild‐type (WT), SOD1‐knockout [SOD1(–/–)] and GPX1‐knockout [GPX1(–/–)] female mice (9‐wk old, n = 7–8 per genotype) to assay for bone enzyme activities and mechanical properties in three point bending. Prior to testing, all mice were fed a torula yeast diet supplemented with 0.4 mg Se/kg as sodium selenite. Compared with the WT mice, SOD1(–/–) mice displayed a series of reductions (p < 0.05): 24% in body mass, 8% in femoral length, 43% in femoral structural strength, and 32% in bending stiffness. When differences in body size were accounted for, femoral failure moment in SOD1(–/–) mice remained lower (p < 0.05) than that of WT. Femoral tartrate resistant acid phosphatase activity in SOD1(–/–) was 47% greater (p < 0.05) than the WT. In contrast, GPX1(–/–) mice showed no significant differences in femoral mechanical properties from those of WT mice. In conclusion, knockout of SOD1 exerted a greater impact on femoral mechanical characteristics than that of GPX1 in growing mice.