KMnO4–Octylphenyl Polygylcol, Ether Chemiluminescence System for Flow Injection Analysis of Uric Acid in Urine
✍ Scribed by Zheng Li; Manliang Feng; Jiuru Lu
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 66 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0026-265X
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✦ Synopsis
A fast and simple KMnO 4 -octylphenyl polygylcol ether (OP) chemiluminescence system for flow injection analysis of uric acid is described. When a mixture of sample and OP is injected into acidic KMnO 4 solution in a flow cell, strong chemiluminescence occurs. The response is linear to the concentration of uric acid in the range 0.10 to 600 mg ml 01 with different permanganate concentrations. The relative standard deviation of the method is between 1.7 and 3.1%. The detection limit is 0.055 mg ml 01 . Most common substances in biological samples did not interfere, except ascorbic acid, which should be spoiled with Fe(III). The results can be obtained within 1 min for each measurement. The recommended method has been successfully tested for determination of uric acid in human urine. Chemiluminescence intensity was found to be remarkably enhanced in the presence of the OP micellar system.