Kinetik des Wachstums und der Riboflavinüberproduktion von Candida guilliermondii in kontinuierlicher Kultur
✍ Scribed by Dipl.-Biol. J. Sauerstein; G. Straube; W. Fritsche
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 390 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
The kinetics of growth, yield, and riboflavin overproduction in continuous culture of Candida guilliermondii were investigated. Iron was the limiting substrate with an influent concentration of 10 μg Fe μ 1^−1^.
The steady state concentration of biomass increases with decreasing dilution rate (D) in the range of D between 0.019 and 0.184 h^−1^.
The production of biomass per glucose (__Y__C~6~H~12~O~6~) decreases from 0.260 to 0.093 with decreasing dilution rate from 0.184 to 0.019 h^−1^. Under conditions of 0 < D ≦ 0.012 h^−1^ the growth is not only limited by substrate iron.
The steady state riboflavin concentration increases with decreasing D, if 0.019 ≦ D ≦ 0.136 h^−1^. The quotient p̄RF/√x demonstrating the amount of produced riboflavin per biomass also increases. Maximum values of specific riboflavin formation rate (k~p~RF) are measured at dilution rates about 0.136 h^−1^.
It is concluded that growth and riboflavin overproduction are connected.