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Kinetics of protease hydrolysis of extended peptide substrates: Measurement by flow-injection analysis

✍ Scribed by Georges L. Chong; Lawrence C. Davis; Gerald R. Reeck

Book ID: 102989408
Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 545 KB
Volume: 186
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90078-n

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✦ Synopsis

A flow-injection analysis (FIA) system was developed to study the enzyme-catalyzed hydrolysis of synthetic peptides, each of which contained one scissile bond. The concentrations of alpha-amino groups in reactions mixtures were determined by FIA with o-phthalaldehyde as a fluorescence reagent. The method allows a rapid, precise, and sensitive determination of kinetic constants for proteases acting on extended peptide substrates.

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