Kinetics of long-term (72 hr) calcium content during mitogen activation of cultured human T lymphocytes
✍ Scribed by C. H. J. Wolff; K. E. O. Åkerman; L. C. Andersson
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 476 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
In vitro stimulation of human blood I m hoc tes with mitogen resulted in an increased intracellular content of Ca per unit cell volume. This increase in Ca2+ content of lectin-activated cells reached a maximum after 24 h r of culture and thereafter slowly declined. Brief treatment of cells at 24 hr of culture with the Ca2+ ionophore A23187 in combination with EGTA resulted in a larger release of Ca2+ from cells in mitogen-stimulated cultures than from cells in control cultures. This indicates that the Ca2+ is accumulated intracellularly but is readily exchangeable. At 24 h r of culture the increase in cellular Ca2+ correlated well with the proliferative response as measured by 3H-thymidine incorporation. Ca2+ influx at 24 and 48 h r of culture was markedly enhanced in the mitogenically stimulated cells as compared either to cells cultured for 1 and 72 h r or cells cultured without mitogen.