Kinetics of glucoamylase hydrolysis of corn starch

✍ Scribed by Jin-Peng Wang; Ai-Wu Zeng; Zhen Liu; Xi-Gang Yuan

• Publisher: Wiley (John Wiley & Sons)
• Year: 2006
• Tongue: English
• Weight: 77 KB
• Volume: 81
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.1435

✦ Synopsis

Abstract

Corn starch was hydrolysed by glucoamylase (EC 3.2.1.3, 1,4‐a‐D‐glucan glucohydrolase) in a chemostat; 106 g L^−1^ reducing sugar was produced at 40 °C after 46 h, whereas only 42 g L^−1^ was produced at 30 °C. When corn starch concentration was increased from 64.6 g L^−1^ to 161.5 g L^−1^, the amount of reducing sugar produced increased with the initial substrate concentration. The studies show that there is a product concentration (p~0~) above which product inhibition becomes apparent. A new model for starch hydrolysis considering the product inhibition point is presented. Copyright © 2006 Society of Chemical Industry