Kinetics of enzymic hydrolysis of malto-oligosaccharides: A comparison with acid hydrolysis

✍ Scribed by Pier Luigi Beltrame; Paolo Carniti; Bonaventura Focher; Annamaria Marzetti; Cataldo Santoro; Alain Heyraud; Marguerite Rinaudo

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 694 KB
• Volume: 166
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(87)80045-9

✦ Synopsis

The hydrolysis of malto-oligosaccharides G3-G6 catalysed by porcine pancreatic alpha-amylase was investigated kinetically at 25". Kinetic parameters ~~esponding to different positions of enzymic attack were determined and product inhibition was evaluated. The enzymic hydrolysis was compared in terms of reaction rate and pattern of action with hydrolysis in 0.1~ H,SO, at 7@. Mathematical models for the mechanism of hydrolysis were developed and a good rationalisation of the experimental results was achieved. P. L.. RELTRAME et al.

kinetic study and now report on the enzymic and acid hydrolysis of GJ-G,. Some preliminary results of this study have been described".

Recently, the initial rates of hydrolysis of several malto-oligosaccharides catalysed by PPA have been measured, and K,,, and k, values have been obtained and correlated with chain length]".