Kinetics of enzymatic hydrolysis of cellulose: Analytical description of a mechanistic model

✍ Scribed by M. Okazaki; M. Moo-Young

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 952 KB
• Volume: 20
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260200503

✦ Synopsis

Abstract

A generalized mechanistic model for the enzymatic hydrolysis of cellulose is developed and expressed mathematically. The model is based on Michaelis–Menten‐type kinetics for concurrent random and endwise attack of the substrate involving end‐product inhibitions and three types of enzymes: an endo‐β;‐1,4‐glucanase, an exo‐β‐1,4‐glucanase, and β‐glucosidase. Basic parameters of the model which can explain synergistic and other effects observed experimentally are quantified and discussed. It is shown that cellulose degradation kinetics are expected to be strongly affected by the ratio of endo‐ to exocellulases in the reaction mixture as indicated by previous experimental data, and the substrate degree of polymerization, a factor not fully appreciated in previous studies, which appear to be overridingly important in many practical cases.