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Kinetics of DNA renaturation catalyzed by RecA protein of Escherichia coli

โœ Scribed by McEntee, Kevin

Book ID
126402410
Publisher
American Chemical Society
Year
1985
Tongue
English
Weight
905 KB
Volume
24
Category
Article
ISSN
0006-2960

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A temperature sensitive RecA protein of
A temperature sensitive RecA protein of Escherichia coli
โœ Hickson, Ian D. ;Gordon, Robert L. ;Tomkinson, Alan E. ;Emmerson, Peter T. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 470 KB

The temperature sensitive allele recA200 has been cloned into the multiple copy number plasmid pBR322 and the gene product isolated. The purified RecA200 protein is temperature sensitive in ability to cleave the phage lambda and LexA repressors in vitro and also in ability to promote a successful se