Enzyme Kinetics Determined Using Calorim
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Matthew J. Todd; Javier Gomez
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Article
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2001
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Elsevier Science
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English
⚖ 119 KB
Two techniques for determining enzyme kinetic constants using isothermal titration microcalorimetry are presented. The methods are based on the proportionality between the rate of a reaction and the thermal power (heat/time) generated. (i) An enzyme can be titrated with increasing amounts of substra