Kinetic study on the esterification of geraniol and acetic acid in organic solvents using surfactant-coated lipase
✍ Scribed by Shih-Yow Huang; Hsiao-Li Chang
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1999
- Tongue
- English
- Weight
- 169 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Surfactant-coated lipase (SCL) was prepared by coating the surface of Candida cylindracea lipase with the non-ionic surfactant, Span 85. SCL catalyzed the esteriücation of geraniol and acetic acid. The ester product, geranyl acetate, is an important component of ýavor and fragrance compounds. The SCL prepared in this work had low solubility in organic solvent, unlike those prepared by other investigators. A kinetic study of the mechanism of the lipolytic esteriücation and the reaction rate for facilitating the scale-up of the reactors indicated a ping-pong bi bi model with competitive substrate inhibition by acetic acid and dead-end inhibition by acetic acid. Experimental data agreed with the proposed model. Kinetic parameters, and were determined which K i , K m(AA) , K m(GOH) V max , visualized the reaction mechanism of the SCL esteriücation.