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Kinetic study of CD34+ cells during peripheral blood stem cell collections

✍ Scribed by P. Bojko; W. Stellberg; C. Küdde; M. Scharifi; M. Herrmann; S. Mayer; A. Harstrick; S. Seeber

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
76 KB
Volume
14
Category
Article
ISSN
0733-2459

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✦ Synopsis

The impact of the separated volume on the yield of CD34+ cells during peripheral blood stem cell collections (PBSCC) remains controversial. We therefore studied the CD34+ cell concentration in the peripheral blood of patients (pts) during PBSCC as well as the total amount of CD34+ cells collected after each blood volume (BV) processed and engraftment data for each cycle of high dose chemotherapy (HD Ctx).

A total of 21 PBSCC from 20 patients with different malignancies were analyzed. Stem cells were mobilized by chemotherapy and G-CSF (14 pts) or GM-CSF (6 pts). Samples from the pts peripheral blood and the collection bag were taken after each BV processed and analyzed for CD34+ cells, WBC, platelets (plt), and hemoglobin (Hb). The total volume processed was two to five times the pts calculated BV (mean value 17.4 L, range 9.0-24.0 L). Sixteen pts could be evaluated for engraftment.

The mean peripheral blood CD34+ cell count was 116±103.5/l at the start of PBSCC and decreased to 57±61.6/l after processing of four times the pts BV. The mean number of CD34+ cells collected after each BV was 2.3±2.4, 5.8±5.2, 8.5±7.2, and 11.8±10.3×10 6 per kg body weight, respectively. The mean plt count decreased by 53±40.2/nl, Hb by 1.±0.5 g/dl and WBC by 0.±6.1/nl after separation of 4 BV. All but two pts reached the target value of 1.5 × 10 6 CD34+ cells/kg body weight and planned cycle of HD Ctx with 1 PBSCC. All pts engrafted and reached neutrophils>500/l and plt>20,000/l at a median of 11 and 13 days, respectively.

We could demonstrate, that the yield of CD34+ cells during PBSCC increased continuously with the volume of the separated BV and that up to 5× the patients' BV could be processed safely without serious side effects. Most pts had to undergo only 1 PBSCC to collect sufficient numbers of CD34+ cells to support sequential courses of HD Ctx without delayed engraftment.

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