Purification of guanosine triphosphate c
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Rudolf L. Then
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Article
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1979
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Elsevier Science
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English
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Folate was coupled t,o AH-Sepharose 4B, the gel poured into small columns, and the Sepharose-bound folate reduced in situ to dihydrofolate by dithionite/ascorbate at pH 6 to 7. The dihydrofolate-Sepharose column was used to purify guanosine triphosphate cyclohydrolase I (EC 3.5.4.16) and dihydrofola