๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Kinetic studies of sepharose-and CH-sepharose-immobilized dihydrofolate reductase

โœ Scribed by Faizy Ahmed; R. Bruce Dunlap


Book ID
102766645
Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
569 KB
Volume
26
Category
Article
ISSN
0006-3592

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๐Ÿ“œ SIMILAR VOLUMES


Purification of guanosine triphosphate c
โœ Rudolf L. Then ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 575 KB

Folate was coupled t,o AH-Sepharose 4B, the gel poured into small columns, and the Sepharose-bound folate reduced in situ to dihydrofolate by dithionite/ascorbate at pH 6 to 7. The dihydrofolate-Sepharose column was used to purify guanosine triphosphate cyclohydrolase I (EC 3.5.4.16) and dihydrofola