Kinetic studies of peptide bond formation. Effect of chloramphenicol

✍ Scribed by Rafael Fernandez-Muñoz; David Vazquez

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 260 KB
• Volume: 1
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00357584

✦ Synopsis

There might be an undetermined order in the interaction of the substrates with the ribosomes in the reaction of CACCA-Leu-Ac with puromycin to form Ac-Leu-puromycin and CACCA ('fragment reaction'). Km 0°=6×10(-4) M for the puromycin · ribosome interaction. Chloramphenicol totally blocks the 'fragment reaction' as a consequence of a single interaction with the ribosome of Kd 0°=2.2×10(-6) M. The inhibition by chloramphenicol of the 'fragment reaction' is mixed competitive for puromycin.