Kinetic properties of gel-immobilized acid phosphatase in a tubular membrane reactor

✍ Scribed by F. Alfani; Donatella Albanesi; Maria Cantarella; V. Scardi

• Book ID: 104632485
• Publisher: Springer Netherlands
• Year: 1981
• Tongue: English
• Weight: 238 KB
• Volume: 3
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00127400

✦ Synopsis

Acid phosphatase has been immobilized onto the internal surface of tubular ultrafiltration membranes by two different methods, namely copolymerization/gelation and co-gelation. Rate parameters for p-nitrophenyl phosphate hydrolysis by the enzyme in both gel-immobilization conditions have been determined and compared to the corresponding values obtained in previous work using a flat ultrafiltration membrane. Results indicate that the kinetic properties of the enzyme seems not substantially modified by the membrane geometry; however, for industrial purposes an enz3~e reactor equipped with tubular membranes should be preferred.