Kinetic model for simultaneous cell disruption and aqueous two-phase extraction

✍ Scribed by Bingsheng Chang; Zhiguo Su

• Publisher: Wiley (John Wiley & Sons)
• Year: 2006
• Tongue: English
• Weight: 140 KB
• Volume: 81
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.1427

✦ Synopsis

Abstract

A process of simultaneous cell disruption and aqueous two‐phase extraction demonstrated improved product yield and selectivity compared with the traditional process of cell disruption followed by extraction. Addition of aqueous two‐phase components did not decrease the efficiency of cell disruption. Moreover, ADH, LDH and G6PDH recovery was enhanced in this new process with recovery ratios of 97%, 93% and 95%, respectively. Cell disruption kinetics were established based on a new mechanism that was different from traditional first‐order kinetics, consisting of the release of intracellular proteins and enzymes, the denaturation and subsequent renaturation of these proteins that may be due to the protection of the aqueous two‐phase components during the cell disruption process. To account for this, the kinetics parameters were calculated, and the experimental data were regressed. The resulting kinetic model could provide a better fit for the experimental data with a correlation ratio of 99% (for total protein), and led to insights into the differences between the new process and the traditional one. Copyright © 2005 Society of Chemical Industry