Kinetic mechanism of β-xylosidase from Trichoderma reesei QM 9414
✍ Scribed by Marı́a Gómez; Pablo Isorna; Marta Rojo; Pilar Estrada
- Book ID
- 114395678
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 101 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1381-1177
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The separation and purification method of extracellular cellobiase and aryl-8-glucosidase from Trichoderma reesei by means of affinity chromatography on controlled porosity glass activated by 7aminopropyltriethoxysilane and oxiranes are described.
Irreversible thermoinactivation of cellobiohydrolase I from Trichoderma reesei has been analyzed at 70°C and pH 4.8. The time course of thermal inactivation and the dependence of the inactivation rates on protein concentration suggested that aggregation followed by precipitation was the main process
## Siimmary I n this paper we studied the conditions for the production of @-glucosidase from T. reesei QM9414 in batch cultures using milled and sieved wheat straw as sole carbon source. High 8-glucosidase production in the presence of wheat straw, a more realistic substrate than commercial cellu