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Kinetic mechanism of the EcoRI DNA methyltransferase

โœ Scribed by Reich, Norbert O.; Mashhoon, Neda

Book ID
125840059
Publisher
American Chemical Society
Year
1991
Tongue
English
Weight
922 KB
Volume
30
Category
Article
ISSN
0006-2960

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DNA substrate analogs were constructed from poly(dC-dG), M13, and XP12 DNA which do not contain a mixture of types of methylation sites. These were used to distinguish different kinetic mechanisms for maintenance and de novo methylation using a highly purified rat liver DNA (cytosine-5) -methyltrans