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Kinetic Mechanism of Human Hypoxanthine−Guanine Phosphoribosyltransferase: Rapid Phosphoribosyl Transfer Chemistry†

✍ Scribed by Xu, Yiming; Eads, Janina; Sacchettini, James C.; Grubmeyer, Charles


Book ID
111683413
Publisher
American Chemical Society
Year
1997
Tongue
English
Weight
402 KB
Volume
36
Category
Article
ISSN
0006-2960

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A micromodification of the method of HGPRT and APRT assay is described, which measures the incorporation of 14C hypoxanthine and 14C adenine into cultured skin fibroblasts and amniotic cells grown on microtiter plates. Only about 10000 cells are needed per assay. By this method HGPRT deficient cells