Kinetic Locking-On Strategy and Auxiliary Tactics for Bioaffinity Purification of NAD(P)+-Dependent Dehydrogenases

✍ Scribed by Patricia Mulcahy; Martina O'Flaherty

• Book ID: 102561114
• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 284 KB
• Volume: 299
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2001.5391

✦ Synopsis

GENERAL

1 Reviews under this heading have been defined as articles that propose methods whose development has reached the stage of being prospective, that is, are ready and likely to come about. 2 Abbreviations used: ADH, alcohol dehydrogenase (EC 1.1.1.1-2); YADH, Saccharomyces cerevisiae alcohol dehydrogenase (EC 1.1.1.1); HLADH, equine liver alcohol dehydrogenase (EC 1.1.1.1); TBADH, Thermoanaerobacter brockii alcohol dehydrogenase (EC 1.1.1.2); GDH, glutamate dehydrogenase from bovine liver (EC 1.4.1.3) and Candida utilis (EC 1.4.1.4); L-LDH, L-lactate dehydrogenase (EC 1.1.1.27); MDH, malate dehydrogenase (EC 1.1.1.37); PheADH, phenylalanine dehydrogenase (EC 1.4.1.20); AlaDH, alanine dehydrogenase (EC 1.4.1.1); G-6-P-DH, glucose-6-phosphate dehydrogenase (EC 1.1.1.49); BSA, bovine serum albumin; NAD ϩ , ␤-nicotinamide adenine dinucleotide; NADP ϩ , ␤-nicotinamide adenine dinucleotide phosphate; 5ЈAMP, adenosine 5Ј-monophosphate; 2Ј5ЈADP, adenosine 2Ј,5Ј-diphosphate; NMN, nicotinamide mononucleotide; GTP, guanosine 5Ј triphosphate; EDAC, 1-ethyl-3(3-dimethylaminopropyl)carbodiimide.