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Kinetic fluorimetric determination of histidine, histamine and their mixtures

✍ Scribed by M.C. Gutierrez; A. Gomez-Hens; M. Valcárcel

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
449 KB
Volume
185
Category
Article
ISSN
0003-2670

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✦ Synopsis

Kinetic fluorimetric methods for the determination of histidine and histamine, both separately and in mixtures, are described; the methods are baaed on the accelerating effect of these compounds on the oxidation of 1,1,3-tricyano-2-amino-1-propene by hydrogen peroxide in the presence of copper. A differential rate method is used for resolution of mixtures of these compounds (lo-" M level), in which synergic effects are taken into account. The relative standard deviations for histidine and histamine are 1.4% and 2.1%, respectively, for separate determinations but 4.0% and 3.4%, respectively, for determinations of mixtures. Histamine/histidine ratios between 1:l and 16:l are satisfactorily resolved.

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