Kinetic Evidence for Incorrectly Folded Intermediate States in the Refolding of Denatured Proteins
โ Scribed by TANFORD, CHARLES; IKAI, ATSUSHI
- Book ID
- 109677158
- Publisher
- Nature Publishing Group
- Year
- 1971
- Tongue
- English
- Weight
- 263 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/230100a0
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR ## SYNOPSIS Binding of the hydrophobic fluorescent probe, 1-anilino-naphthalene-8-sulfonate ( ANS) , to synthetic polypeptides and proteins with a different structural organization has been studied. It has been shown that ANS has a mu
Elucidating the properties of the denatured state of proteins under conditions relevant for their folding is a key factor in understanding the folding process. We show that a peptide corresponding to residues 111-120 of human โฃ-lactalbumin has a pronounced propensity to adopt nonnative structure in
## Abstract The kinetics of the aqueous cleavage of __N__โethoxycarbonylphthalimide (NCPH) in CH~3~NHOH buffers of different pH reveals that the cleavage follows the general irreversible consecutive reaction path NCPH $\buildrel{k\_{1\,{\rm obs}}}\over\longrightarrow$ ENMBC $\buildrel{k\_{2\,{\rm