Kinetic discrimination of tryptophan residues of glucoamylase from Rhizopus niveus by fast chemical modification with N-bromosuccinimide

A pH-induced change in state around acti

A pH-induced change in state around active-site tryptophan residues of Rhizopus niveus glucoamylase, detected by stopped-flow studies of chemical modification with N-bromosuccinimide

✍ Masatake Ohnishi; Yuki Nakamura; Masako Murata-Nakai; Keitaro Hiromi 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 541 KB

At pH 4.5 (the optimum), stopped-flow studies of the chemical modification with N-bromosuccinimide (NBS) of the tryptophan residues of glucoamylase from Rhizopus niveus clearly show reaction curves having two phises, fast and slow, in which two separate types of Trp residues are respectively involve