✦ LIBER ✦

Kinetic characterization of the extracellular xylanases of Thermomonospora sp.

✍ Scribed by D. L. Ristroph; A. E. Humphrey

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 431 KB
Volume: 27
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260270612

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✦ Synopsis

This study deals with characterizing the extracellular xylanases produced by a strain of the thermophilic bacterial genus Thermomonospora. Supernatant from centrifuged fermentation broth was used as a crude enzyme preparation. From pH 5.5 to pH 7.7 the temperature optimum based on a 10-min assay of activity was 80°C. The crude enzyme had a half-life of approximately 1 month when stored at 55°C at pH 6.5. The enzyme produced a mixture of xylose oligomers from xylan, with xylobiose occurring in greatest quantity on a molar basis. Only trace quantities of xylose were produced by this hydrolysis.

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