Kinetic characterization of Na+/H+ antiport of Plasmodium falciparum membrane

✍ Scribed by A. Bosia; D. Ghigo; F. Turrini; E. Nissani; G. P. Pescarmona; H. Ginsburg

• Book ID: 102882738
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 879 KB
• Volume: 154
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041540311

✦ Synopsis

Y I Y04, Israel (E.N., H.C.I Intraerythrocytic malaria parasites produce vast amounts of lactic acid through glycolysis. While the egress of lactate is very rapid, the mode of extrusion of H + is not known. The possible involvement of a Nat/Ht antiport in the extrusion of protons across the plasma membrane of Warmodium falciparum has been investigated by using the fluorescent pH probe 6-carboxyfluorescein. The resting cytosolic pH was 7.27 * 0.1 in ring stage parasites and 7.31 ? 0.1 2 in trophozoites.

Spontaneous acidification of parasite cyto5ol was observed in N a ' -free medium