𝔖 Bobbio Scriptorium
✦   LIBER   ✦

KINETIC CHARACTERIZATION OF CALCIUM UPTAKE BY THE RAT LIVER GOLGI APPARATUS

✍ Scribed by Patricio Rojas; Amarilys Surroca; Ariel Orellana; Daniel Wolff

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
110 KB
Volume
24
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

We carried out a kinetic characterization of the Ca^2+^active transport in the rat liver Golgi Apparatus (GA) membrane. Calcium accumulation by vesicles of a GA enriched fraction was found to be a function of both Ca^2+^and ATP‐Mg concentrations, it was inhibited by 2μm thapsigargin but not stimulated by 3μm calmodulin. The kinetic parameter values obtained for the GA Ca^2+^pump were: J~max~of 3.96nmol/mg min, K~m~for Ca^2+^of 0.150μm and two K~m~'s for ATP of 1.14μm and 519μm. These results were almost identical to those obtained for the endoplasmic reticulum (ER) fraction, indicating that the GA Ca^2+^pump is a sarco/endoplasmic reticulum (SERCA) P‐type, analogous—if not identical—to that present in the ER.

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