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Kinetic, Binding, and NMR Studies of Perdeuterated Yeast Phosphoglycerate Kinase and Its Interactions with Substrates

✍ Scribed by C.G. Shibata; J.D. Gregory; B.S. Gerhardt; E.H. Serpersu


Book ID
115565609
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
525 KB
Volume
319
Category
Article
ISSN
0003-9861

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Fully deuterated yeast phosphoglycerate kinase ([2H]PGK) was prepared biosynthetically with only histidine side chains of normal (1H) isotopic composition. The 1H NMR spectrum of this enzyme ([1H]His[2H]PGK) showed that the histidine side chains are clearly visible as sharp signals. Thus detailed st