Proton NMR Studies of a Large Protein. p
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K.M. Pappu; E.H. Serpersu
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Article
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1994
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Elsevier Science
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English
β 733 KB
Fully deuterated yeast phosphoglycerate kinase ([2H]PGK) was prepared biosynthetically with only histidine side chains of normal (1H) isotopic composition. The 1H NMR spectrum of this enzyme ([1H]His[2H]PGK) showed that the histidine side chains are clearly visible as sharp signals. Thus detailed st