Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors
✍ Scribed by Jianfeng Xu; Jian Xie; Aiming Han; Pusun Feng; Zhiguo Su
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1998
- Tongue
- English
- Weight
- 283 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Compact callus aggregates (CCA) of Rhodiola sachalinensis, spherical, smooth-surfaced clumps of 2È8 mm in diameter displaying some level of tissue di †erentiation, were successfully cultured in 10 dm3 and 100 dm3 air-lift reactors. High salidroside yields of 60É0 mg dm~3 were obtained, which were 10-fold the dispersed cell cultures. The salidroside accumulation was found to be growthassociated due to the di †erentiated structure of CCA. No "foamingÏ was observed since the broth remained almost clear throughout the culture cycle. The size of CCA conformed to normal distribution with average diameters varying from 3É1 mm to 3É6 mm during the culture. The depositing velocity of CCA in culture broth was small enough to be readily retained in suspension, therefore avoiding the clogging of the reactors. The signiÐcant increase in solid hold-up of the culture system was suggested to contribute to the variation of during the k L a culture. SCI ( 1998