Purification and properties of NAD+-depe
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Dr. Mitsugi Iida; Shin'Ichi Ohtsuki; Shigeru Mineki
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Article
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1993
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John Wiley and Sons
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English
β 550 KB
Formate: NAD+ oxidoreductase (EC 1.2.1.2) with high affinity to formate was purified from a facultative formate-utilizing Agrobacterium sp. MIL 1052. From a cell extract, the enzyme was purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, Sephadex (3-2