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Kinetic and structural properties of Nad-Dependent bacterial formate dehydrogenase

✍ Scribed by A. M. Egorov; V. O. Popov; I. V. Berezin; U. V. Rodionov


Book ID
112964634
Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
835 KB
Volume
5
Category
Article
ISSN
0273-2289

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Purification and properties of NAD+-depe
✍ Dr. Mitsugi Iida; Shin'Ichi Ohtsuki; Shigeru Mineki πŸ“‚ Article πŸ“… 1993 πŸ› John Wiley and Sons 🌐 English βš– 550 KB

Formate: NAD+ oxidoreductase (EC 1.2.1.2) with high affinity to formate was purified from a facultative formate-utilizing Agrobacterium sp. MIL 1052. From a cell extract, the enzyme was purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, Sephadex (3-2