Kinetic Analysis of cAMP-Dependent Protein Kinase: Mutations at Histidine 87 Affect Peptide Binding and pH Dependence

E230Q mutation of the catalytic subunit

E230Q mutation of the catalytic subunit of cAMP-dependent protein kinase affects local structure and the binding of peptide inhibitor

✍ Man-Un Ung; Benzhuo Lu; J. A. McCammon 📂 Article 📅 2006 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 484 KB 👁 2 views

## Abstract The active site of the mammalian cAMP‐dependent protein kinase catalytic subunit (C‐subunit) has a cluster of nonconserved acidic residues—Glu127, Glu170, Glu203, Glu230, and Asp241—that are crucial for substrate recognition and binding. Studies have shown that the Glu230 to Gln mutant