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Kinesin Generates a Nucleotide-Dependent Distortion of the Microtubule Lattice

✍ Scribed by Sindelar, Charles V.; Downing, Kenneth H.


Book ID
119208618
Publisher
Biophysical Society
Year
2011
Tongue
English
Weight
34 KB
Volume
100
Category
Article
ISSN
0006-3495

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We studied the effect of alkaline-earth metal ions on the kinesin-driven gliding of microtubules, using a narrow glass chamber enabling the exchange of buffer components without interrupting microscopic observation. Under standard conditions (0.5 mM Mg 21 ), microtubules were found to glide at a mea