✦ LIBER ✦

Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?

✍ Scribed by Kural, C.

Book ID: 111876397
Publisher: American Association for the Advancement of Science
Year: 2005
Tongue: English
Weight: 257 KB
Volume: 308
Category: Article
ISSN: 0036-8075
DOI: 10.1126/science.1108408

No coin nor oath required. For personal study only.

✦ Synopsis

We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)–tagged peroxisome in cultured
Drosophila
S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ∼8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.