Killing lung cancer cells at cell-cycle phase by a new indium-111-bleomycin complex

✍ Scribed by De-Yan Hou; Jose V. Ordonez; Richard J. Cross; Douglas D. Ross; Yosh Maruyama

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 489 KB
• Volume: 40
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930400203

✦ Synopsis

The efficacy of killing small cell lung cancer (SCLC) cells at the G I , S, and G2-M phase of the cell-cycle by a new "'In-bleomycin complex (' ' 'In-BLMC) was investigated. SCLC cells (N417, H526, H209) were synchronized by double thymidine block and assessed by DNA content with flow cytometry, and the period for the maximal accumulation of cells in S, GI, or G2-M phase was determined. Cells in different cell cycle phases were exposed to 0.9% NaCl, BLM, or "'In-BLMC for 1 hour and observed for colony formation. The survival of H526 cells treated with "'In-BLMC was 71 % (for enriched S phase), 46% (GI), and 31 % (Gz-M). For N417 cells, it was 25% (S), 20% (GI), and 8% (G2-M) for '''In-BLMC and 18%% (S), 33% (GI), and 10% (G2-M) for BLM. These results indicated that SCLC cells in G2-M were most sensitive and those in S phase were least sensitive to "'In-BLMC; cells in G1 phase were the least sensitive to BLM. ~ ~ ~ ~~ KEY WORDS: "'indium, bleomycin, cancer, cell cycle

' "InC13 (Medi-Physics, Richmond, CA), received in 0.45-0.9% NaCl solution adjusted to pH 1-3 with HCl, was mixed with 1.0 mg solid BLM or with BLM dis-