𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Keratinocyte growth factor protects murine hepatocytes from tumor necrosis factor–induced apoptosis in vivo and in vitro

✍ Scribed by Giorgio Senaldi; Christine L. Shaklee; Bernadett Simon; Christopher G. Rowan; David L. Lacey; Thomas Hartung

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
324 KB
Volume
27
Category
Article
ISSN
0270-9139

No coin nor oath required. For personal study only.

✦ Synopsis

Keratinocyte growth factor (KGF) promotes epithelial growth and differentiation and has potent effects on the liver. The coinjection of lipopolysaccharide (LPS) and D-galactosamine (GalN) results in hepatic failure in mice. Mechanistically, LPS-induced tumor necrosis factor (TNF) triggers hepatocyte apoptosis, which is enhanced by GalNarrested transcription. Similarly, the combination of TNF and actinomycin D (ActD) causes hepatocyte apoptosis in vitro. We studied the effect of KGF on LPS and GalNinduced hepatic failure in vivo and on TNF-and ActDinduced hepatocyte apoptosis in vitro, where it was compared with those of hepatic growth factor (HGF) and epidermal growth factor (EGF). Mice treated with human recombinant KGF (1 mg/kg subcutaneously) 24 hours before intraperitoneal coinjection of LPS and GalN sustained prolonged survival compared with control mice, although overall mortality was not changed. The counts of apoptotic hepatocytes, serum alanine and aspartate transaminases, and DNA fragments in the cytosolic fraction of liver homogenates were higher in control mice than in treated mice 6 hours after LPS and GalN coinjection, before any mortality occurred. In vitro, hepatocytes pretreated with KGF exhibited reduced TNF-and ActD-induced cell damage and DNA fragmentation, similar to hepatocytes pretreated with HGF and EGF. In conclusion, KGF prolongs survival during LPS-and GalN-induced hepatic failure by temporarily protecting hepatocytes against apoptosis. It also protects hepatocytes in vitro against TNF-and ActDinduced apoptosis.

📜 SIMILAR VOLUMES

Nerve growth factor inhibits apoptosis i
Nerve growth factor inhibits apoptosis induced by tumor necrosis factor in PC12 cells
✍ Ronit Haviv; Reuven Stein 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB 👁 2 views

Tumor necrosis factor-␣ (TNF␣) may play a role in at least some of the neuronal death that occurs following brain insults or in neurodegenerative diseases. It is therefore important to characterize the mechanism underlying apoptosis induced by TNF␣ in neuronal cells and to identify factors capable o

Interleukin-1α, tumor necrosis factor-α,
Interleukin-1α, tumor necrosis factor-α, and interleukin-12 secreted by zinc-induced murine macrophages in vivo and in vitro
✍ M.D. Lastra; A.E. Aguilar; M.A. Cabañas; R. Hernandez; K. Humanez; R. Pastelin 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 172 KB

## Abstract Oral treatment of zinc modulates cytokine secretion during the gestation, lactation, and weaning periods. We used a experimental mice model of zinc supplementation during the perinatal stages to study the effects of this ion over the production of interleukin IL‐1α, IL‐12, and tumor nec

Tumor necrosis factor-α–induced apoptosi
Tumor necrosis factor-α–induced apoptosis is associated with suppression of insulin-like growth factor binding protein-5 secretion in differentiating murine skeletal myoblasts
✍ Kate A. Meadows; Jeff M.P. Holly; Claire E.H. Stewart 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 264 KB 👁 1 views

Wasting of muscle and fat during cachexia exceeds that explained by reduced food intake alone. This wasting may result from an imbalanced cytokine environment, which could lead to increased protein catabolism. Supporting this, tumor necrosis factor-alpha (TNF-alpha) is raised in several animal model

Insulin-like growth factor I is the key
Insulin-like growth factor I is the key growth factor in serum that protects neuroblastoma cells from hyperosmotic-induced apoptosis
✍ Cynthia M. Van Golen; Eva L. Feldman 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 249 KB 👁 2 views

Neuroblastoma is a childhood tumor of the peripheral nervous system that remains largely uncurable by conventional methods. Mannitol induces apoptosis in neuroblastoma cell types and insulin-like growth factor I (IGF-I) protects these cells from hyperosmotic-induced apoptosis by affecting apoptosis-

Heme oxygenase-1 protects against apopto
Heme oxygenase-1 protects against apoptosis induced by tumor necrosis factor-α and cycloheximide in papillary thyroid carcinoma cells
✍ George G. Chen; Z.M. Liu; A.C. Vlantis; G.M.K. Tse; B.C.H. Leung; C.A. van Hasse 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 331 KB

## Abstract Heme oxygenase‐1 (HO‐1) plays a role in the resistance to apoptosis of several types of cells, but its role in the development of thyroid cancer is unknown. In this study, we investigated the regulation of HO‐1 in human papillary thyroid carcinoma cells (KAT5). The results show that HO‐

Transforming growth factor-β and tumor n
Transforming growth factor-β and tumor necrosis factor-α cooperate to induce apoptosis in the oligodendroglial cell line OLI-neu
✍ Norbert Schuster; Herdis Bender; Oliver G. Rössler; Anja Philippi; Nicole Dünker 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB

## Abstract As shown previously, transforming growth factor‐β (TGF‐β) plays an important role during the period of developmental cell death in the nervous system. As with neurons, oligodendrocytes are generated in excess and eliminated by apoptosis. The present study was aimed at investigating the