KCTD10 interacts with proliferating cell nuclear antigen and its down-regulation could inhibit cell proliferation

✍ Scribed by Yibo Wang; Yi Zheng; Fang Luo; Xiaohan Fan; Jinxing Chen; Channa Zhang; Rutai Hui

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 131 KB
• Volume: 106
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.22026

✦ Synopsis

Abstract

A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel tetramerisation domain‐containing 10) was cloned from our 5300 EST database of human aorta cDNA library. Computational analysis showed that KCTD10 cDNA is 2,638 bp long, encoding 313 amino acids with a proliferating cell nuclear antigen binding motif, mapped to chromosome 12q24.11 with 7 exons, ubiquitously expressed in all 12 tested normal tissues and 7 of 8 tested tumor cell lines from MTN membranes by Northern blot. Nuclear localization of KCTD10 was observed in A549 cells. Yeast two‐hybrid analysis and immunoprecipitation assay showed that KCTD10 can interact with PCNA. In A549 cells, KCTD10 down‐regulation could inhibit cell proliferation, but its over‐expression could not influence cell proliferation. The results suggest that KCTD10 may be associated with DNA synthesis and cell proliferation. J. Cell. Biochem. 106: 409–413, 2009. © 2009 Wiley‐Liss, Inc.