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Karyotyping of bone-marrow cells in hematologic diseases

✍ Scribed by H. G. Morse; J. R. Humbert; J. J. Hutter; A. Robinson

Publisher
Springer
Year
1977
Tongue
English
Weight
349 KB
Volume
37
Category
Article
ISSN
0340-6717

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✦ Synopsis

Cultures of bone-marrow cells incubated for 5 h at 4 degrees had cells in metaphase with elongated chromosomes which were easily Giemsa banded. Colcemid was not necessary for metaphase arrest at this temperature. This technique made possible routine karyotyping of patients with leukemia and other hematological diseases in which the detection of aberrations was otherwise difficult.

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