Kainic acid?induced excitotoxicity is associated with a complex c-Fos and c-Jun response which does not preclude either cell death or survival
✍ Scribed by Pozas, Esther ;Ballabriga, Jordi ;Planas, Anna M. ;Ferrer, Isidro
- Book ID
- 101258287
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 372 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
c-fos and c-jun mRNA induction ern blots to c-Fos showed a double band at p62 in and c-Fos and c-Jun protein expression were examsamples containing the hippocampus and entorhinal ined in the brains of adult rats subjected to systemic and piriform cortices (hip samples) and in samples kainic acid (KA) injection at convulsant doses. Induccontaining the neocortex (cortex samples). The upper tion of c-fos and c-jun mRNA, as seen with in situ band was abolished following preincubation of the hybridization, occurred in the piriform and entorhinal samples with alkaline phosphatase, thus suggesting ccortices, neocortex, amygdala, hippocampus, dentate Fos phosphorylation. Western blots to c-Jun (AB-1) gyrus, and discrete thalamic nuclei. This was followed showed a single band at about p39 in hip and cortex. by c-Fos protein expression, as revealed with immuno-However, Western blots to c-Jun/AP1 (N) identified histochemistry, in the same regions. However, the distwo bands. One band at about p39 was seen in control tribution of c-Jun protein expression differed derats and the cortex of KA-treated rats. Another band pending on the antibody used. The distribution of cells at p26 was observed only in hip samples of KA-treated immunostained with the antibody c-Jun (AB-1) was rats. In addition, decreased c-Jun N-terminal kinase similar to that of c-jun mRNA, but the distribution of 1 (JNK-1) expression, as revealed on Western blots, cells immunostained with the antibody c-Jun/AP1 (N) was coincidental with the appearance of the p26 cwas restricted to a few neurons in the pyramidal cell Jun-immunoreactive band in KA-treated rats. These layer of CA1 and CA3, layer II of the piriform and results show that c-Fos and different Jun-related antientorhinal cortices, basal amygdala, and discrete thalgens are expressed following KA excitotoxicity, and amic nuclei. Although the regional distribution of cthat posttranslational modifications involving phos-Fos-and c-Jun-immunoreactive cells in the hippophorylation of c-Fos and Jun(s) may occur following campus, layer II of the entorhinal and piriform corti-KA injection. These results also stress the necessity ces, basal amygdala, and discrete thalamic nuclei of examining the composition of Fos and Jun-related matched the distribution of cells committed to dying, antigens and the metabolic state of Fos and Jun(s) c-Fos-and c-Jun-immunoreactive cells in the neoin different experimental models of nervous system cortex and dentate gyrus survived. Therefore, the injury.