K+ transport and energetics in Kir6.2−/− mouse hearts assessed by 87Rb and 31P magnetic resonance and optical spectroscopy
✍ Scribed by Olga Jilkina; Bozena Kuzio; John Rendell; Bo Xiang; Valery V. Kupriyanov
- Book ID
- 116983882
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 60 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0022-2828
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## Abstract We studied the fluxes of a potassium congener (Rb^+^) in mouse hearts by ^87^Rb MRS at 8.4T. The hearts were loaded with Rb^+^ by perfusion with Krebs‐Henseleit buffer, in which 50% of K^+^ was substituted with Rb^+^. We initiated Rb^+^ efflux by changing the perfusion medium to Rb^+^‐f
An experimental model was developed to evaluate the effects of activators and inhibitors of K(ATP) channels on unidirectional K+ fluxes in the whole heart. Isolated rat hearts perfused in the Langendorff mode were equilibrated with Pi-free Krebs-Henseleit buffer (KH buffer) containing 0.94-2.14 mM R
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