K-series centrifuges: IV. Measurement and control of temperature

✍ Scribed by J.N. Brantley; D.D. Willis; J.P. Breillatt; R.F. Gibson; L.C. Patrick; N.G. Anderson

• Publisher: Elsevier Science
• Year: 1970
• Tongue: English
• Weight: 431 KB
• Volume: 36
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(70)90380-5

✦ Synopsis

The K-series centrifuges were initially designed for the isolation of influenza virus from chorioallant,oic fluid (I, 2). Temperat,urc control is not critical for the preparation of influenza vaccine; therefore, provisions for it were minimal. Aqueous coolant in a cont'ninment system was circulated around the seals, cooled oil was passed through 'the bearings, and cold brine was passed through a refrigera.tion jacket lining the rotor chatiber (2).

The use of the K-series centrifuges has been expand4 beyond vaccine production into the field of serum and subcellular particle fractionation by tht design and fabrication of rotors K-III through K-XI (t.0 be descrilbed in later publications).

For many of these pcparations it is necessary to 'control the temperature of the rotor and the 'continuous-flow sample stream near 3°C in a K-cent,rifuge with a C-t'ype casing (1).