✦ LIBER ✦

K-ras AND p53 MUTATIONS IN HUMAN COLORECTAL ABERRANT CRYPT FOCI

✍ Scribed by LOSI, LORENA; RONCUCCI, LUCA; DI GREGORIO, CARMELA; DE LEON, MAURIZIO PONZ; BENHATTAR, JEAN

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 522 KB
Volume: 178
Category: Article
ISSN: 0022-3417
DOI: 10.1002/(sici)1096-9896(199603)178:3<259::aid-path473>3.0.co;2-4

No coin nor oath required. For personal study only.

✦ Synopsis

Aberrant crypt foci (ACF) are putative precursor lesions of colon cancer, recently identified on the methylene blue-stained mucosal surface of human colon. No mutations in K-vas or p53 genes were found by non-radioactive single-strand conformation polymorphism analysis in 14 ACF collected from five patients. Using the more sensitive method of allele-specific polymerase chain reaction (PCR) for K-vas, 8 of 14 ACF were found to contain K-ras mutations, suggesting that mutated cells are present in minute clones in ACF. No dysplasia was observed in any of the ACF containing a mutated clone. The presence of K-vas mutations in ACF suggests that these lesions occur at a very early stage in human colorectal carcinogenesis.

📜 SIMILAR VOLUMES

Aberrant crypt foci as precursors in col

Aberrant crypt foci as precursors in colorectal cancer progression

✍ Frank A. Orlando; Dongfeng Tan; Juan D. Baltodano; Thaer Khoury; John F. Gibbs; 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 113 KB

K-ras and p53 mutations in hereditary no

K-ras and p53 mutations in hereditary non-polyposis colorectal cancers

✍ Lorena Losi; Maurizio Ponz de Leon; Josef Jiricny; Carmela Di Gregorio; Piero Be 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 57 KB 👁 2 views

Genetic instability related to defective DNA mismatch repair genes may be involved in the pathogenesis of carcinoma in Hereditary Non-Polyposis Colorectal Cancer (HN-PCC). To test that the targets of genetic instability could include critical transforming genes involved in colon tumor progression, w

Mutation analysis of p53, K-ras, and BRA

Mutation analysis of p53, K-ras, and BRAF genes in colorectal cancer progression

✍ Daniele Calistri; Claudia Rengucci; Ian Seymour; Arturo Lattuneddu; Anna maria P 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 162 KB

## Abstract Gene mutations in __APC__, __K‐ras__, and __p53__ are thought to be essential events for colorectal cancer development. Recent data seem to indicate that __K‐ras__ and __p53__ mutations rarely co‐exist in the same tumor, indicating that these alterations do not represent a synergistic e

Increased expression of fatty acid synth

Increased expression of fatty acid synthase in human aberrant crypt foci: Possible target for colorectal cancer prevention

✍ Kathleen E. Kearney; Thomas G. Pretlow; Theresa P. Pretlow 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 French ⚖ 482 KB

## Abstract Aberrant crypt foci (ACF), the earliest identified monoclonal lesions in the colon, provide insights into changes that promote and/or accompany the transformation of normal colonic epithelial cells to colorectal cancer. Fatty acid synthase (FAS), the primary enzyme involved in __de novo

K-ras, p53 mutations, and microsatellite

K-ras, p53 mutations, and microsatellite instability (MSI) in gallbladder cancer

✍ Angelica A. Saetta 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 104 KB

## Abstract Despite the considerable progress in understanding the molecular pathology of carcinogenesis, the genetic mechanisms underlying the development and progression of gallbladder cancer (GC) are poorly understood. The survival of GC patients is generally poor. Therefore, it is very useful t

P53 mutations in colorectal cancer from

P53 mutations in colorectal cancer from northern Iran: Relationships with site of tumor origin, microsatellite instability and K-ras mutations

✍ Mahboobeh Mahdavinia; Faraz Bishehsari; Fabio Verginelli; Albana Cumashi; Rossan 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 209 KB